| Quantity: | |
|---|---|
Clinical relevance – MSU crystal-induced inflammation mimics human gout pathogenesis, including NLRP3 inflammasome activation and IL-1β release.
Two strain options – BALB/c and C57BL/6 models accommodate different genetic backgrounds and experimental needs.
Quantifiable endpoints - foot thickness/joint width, body weight, pain threshold (mechanical allodynia), neutrophil counts in blood and joints, cytokine levels (IL-1β, IFN-γ).
Translational Value – Ideal for testing IL-1 inhibitors (canakinumab, rilonacept), NLRP3 inflammasome inhibitors, colchicine, NSAIDs, and urate-lowering therapies.
IND Ready Packet – Research can be conducted in accordance with GLP principles.
Representative data from our MSU-induced mouse GA model:
C57BL/6 GA model launched by MSU
MSU-induced C57BL/6 GA model (ankle)
MSU-induced BALB/c GA model
• Efficacy testing of anti-inflammatory drugs (IL-1 inhibitors, NLRP3 inflammasome inhibitors, colchicine, NSAIDs, corticosteroids)
• Target validation of IL-1β, NLRP3 and neutrophil recruitment pathways
• Biomarker discovery (IL-1β, neutrophil markers, pain-related mediators)
• Mechanism of action (MOA) studies of anti-gout compounds
• Pharmacological studies to support IND
scope | MSU-induced BALB/c GA model | C57BL/6 GA model launched by MSU |
tension | BALB/c mouse | C57BL/6 mouse |
induction method | Intra- or peri-articular injection of MSU crystals (50-100 μg) | Intra- or peri-articular injection of MSU crystals (50-100 μg) |
study time | 7-14 days after injection | 7-14 days after injection |
critical endpoint | Body weight, foot thickness, pain response (mechanical allodynia), blood neutrophil count, joint neutrophil count, IL-1β, IFN-γ in joints | Joint width, pain threshold, neutrophil count in joint lavage fluid, IL-1β in joint lavage fluid, serial joint imaging |
packet | Raw data, analysis reports, clinical measurements, cytokine ELISA data, flow cytometry files, histology slides (optional), bioinformatics (optional) | |
Q: What is the difference between BALB/c and C57BL/6 GA models?
A: Both strains produce strong MSU-induced inflammation. BALB/c mice generally exhibit a stronger Th2 response, whereas C57BL/6 mice have a more pronounced Th1/neutrophil response. The choice depends on your specific drug mechanism and desired inflammatory profile.
Q: How is pain assessed in these models?
A: Measure paw withdrawal threshold using mechanical allodynia (von Freis) or assess pain by spontaneous pain behavior (guarding, raising).
Q: Can these models be used for IND support studies?
Answer: Yes. Studies can be conducted according to GLP principles for regulatory submissions (FDA, EMA).
Q: Do you offer customized study protocols (e.g., different MSU doses, prevention doses, and treatment doses)?
Answer: Of course. Our scientific team customizes MSU injection regimens, treatment plans, and endpoint analyzes based on your specific drug candidate.