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E tika ana mo te haumanu – Ka whakahoki ano i te hyperuricemia tangata me te nui o te waikawa uric serum, e tika ana mo te ako kauti me te nephropathy waikawa uric.
Ma te mahi miihini - Ko te konupora oxonate te aukati i te uricase (te enzyme e whakaheke ana i te waikawa uric i roto i nga kiore), e whai ana i te paopaotanga waikawa uric a te tangata; ka whakanuia e te adenine te kawenga purine.
Wāhi mutunga ka taea te ine – Ko nga taumata o te waikawa uric serum ka inehia e te karaehe, te whakamatautau enzymatic ranei.
Te uara whakamaoritanga - He pai mo te whakamatautau i nga aukati o te xanthine oxidase (allopurinol, febuxostat), nga rongoa uricosuric, me nga puhui whakaheke urate hou.
Nga kohinga raraunga kua rite IND - Ka taea te whakahaere rangahau i runga i nga tikanga o te GLP.
Ko te PO me te Adenine te Tauira C57BL/6 Hyperuricemia
• Te whakamatautau whaihua o nga aukati xanthine oxidase (allopurinol, febuxostat, topiroxostat)
• Aromatawai i nga rongoa uricosuric (probenecid, benzbromarone, lesinurad)
• Te whakamanatanga whainga mo nga ara paopaotanga waikawa uric
• Te kitenga tohu koiora (waika uric, mahi xanthine oxidase, tohu mahi renal)
• Maatauranga rongoora me te paitini whakaahei IND
Tawhā |
Whakatakotoranga |
Momo/Riki |
C57BL/6 kiore |
Tikanga whakauru |
Te whakahaere waha o te pāhare pāporo oxonate (250–500 mg/kg) + adenine (50–100 mg/kg), ia rā mō te 7–14 rā, he maha tonu te whakakotahi ki te kai purine tiketike. |
Te roanga ako |
1–3 wiki (whakauru + wahanga maimoatanga) |
Nga pito mutunga matua |
Ko nga taumata o te waikawa uric serum, he mea whiriwhiri: serum creatinine, BUN, mahi xanthine oxidase, renal histopathology, urinary uric acid excretion |
| Te whakahaere pai | Allopurinol (xanthine oxidase inhibitor) e wātea ana hei pūhui tohutoro |
Mōkihi Raraunga |
Raraunga mata, purongo tātaritanga, hua matū haumanu, bioinformatics (kwhiria) |
A1: Ka whakatauhia e matou he tauira hyperuricemia pumau i roto i nga kiore C57BL/6 na te whakakotahitanga o te Potassium Oxonate (PO) me te adenine.
A2: Ko te konupora oxonate he aukati i te mahi uricase, engari ko te adenine e whakanui ana i te purine metabolism. Ko te whakakotahitanga ka piki ake te waikawa uric serum me te pee i nga huringa pathological o te hyperuricemia haumanu.
A3: Ko te tohu arotake matua ko te taumata o te waikawa uric serum hei whakaata tika i te paanga whakatauira me te kaha o te mate.
A4: Ka whakahaerehia te PO me te adenine mai i te Ra 0. Ka mahia nga kohinga tauira e pa ana me nga whakamatautau ina oti te tauira.
