| Quantity: | |
|---|---|
Clinically relevant – Recapitulates human DTH reactions with T cell-mediated inflammation, Th1 cytokine profile, and local swelling.
Multiple antigens – Hapten (OXA) and protein antigen (OVA) models available for different research applications.
Quantifiable endpoints – Ear weight and thickness measurements, cytokine profiling (IFN-γ, TNF-α, IL-4) in ear tissue lysate.
Translational value – Ideal for testing immunomodulators targeting T cell responses, including immunosuppressants (corticosteroids, calcineurin inhibitors) and biologics.
IND-ready data packages – Studies can be conducted in accordance with GLP principles.
OXA Induced DTH Model in C57BL/6 Mice
OVA Induced DTH Model in SD rat
• Efficacy testing of immunosuppressants (corticosteroids, calcineurin inhibitors, methotrexate) for T cell-mediated disorders
• Evaluation of biologics targeting T cell activation, co-stimulation, or Th1/Th17 pathways
• Target validation for cell-mediated immunity and inflammatory responses
• Biomarker discovery (Th1/Th2 cytokine profiles, T cell activation markers)
• IND-enabling pharmacology and toxicology studies
Parameter | OXA Induced DTH Model (Mouse) | OVA Induced DTH Model (Rat) |
Species/Strain | C57BL/6 mouse | Sprague-Dawley rat |
Sensitization | Oxazolone (OXA) on abdomen (Day 0) | OVA + CFA (Day 0) |
Challenge | OXA on ear (Day 5) | OVA on ear (Day 7) |
Key endpoints | Ear weight and thickness, cytokine levels in ear tissue lysate (IFN-γ, TNF-α, IL-4), optional: histopathology, immune cell infiltration | |
Data package | Raw data, analysis reports, behavioral data, histology slides (LFB, IHC), image analysis files, bioinformatics (optional) | |
Q: What is the difference between OXA and OVA induced DTH models?
A: OXA is a hapten that induces contact hypersensitivity, directly activating T cells upon skin contact. OVA is a protein antigen requiring processing and presentation, making it more suitable for studying classical DTH responses to protein antigens. The choice depends on your research focus and desired immune pathway.
Q: What are the key similarities with human DTH?
A: Both models exhibit antigen-specific T cell-mediated inflammation, peak response at 24-48h, Th1 cytokine profile (IFN-γ, TNF-α), and local swelling, closely mirroring human DTH reactions such as tuberculin skin test.
Q: Can these models be used for IND-enabling studies?
A: Yes. Studies can be conducted in accordance with GLP principles for regulatory submissions (FDA, EMA).
Q: Do you offer customized study protocols (e.g., different sensitization/challenge timelines, antigen doses)?
A: Absolutely. Our scientific team tailors sensitization protocols, challenge schedules, and endpoint analyses to your specific drug candidate.