| Quantity: | |
|---|---|
Clinical Relevance - Both models closely mimic human neuropathic pain, with mechanical allodynia, rapid onset, and chronic persistence.
Complementary mechanisms - SNI models lack thermal hyperalgesia and can be used to isolate mechanical pain pathways; SNL models produce mechanical and thermal hypersensitivity.
Quantifiable endpoints – mechanical withdrawal threshold (von Frese), thermal withdrawal latency (Hargreaves test, SNL only).
Well characterized and reproducible - a widely used model with an established protocol and high interlaboratory reproducibility.
Translational value – ideal for testing gabapentinoids (gabapentin, pregabalin), sodium channel blockers (lidocaine, carbamazepine) and novel pain therapies.
IND Ready Packet – Research can be conducted in accordance with GLP principles.
SNL surgery induced neuropathic pain model in SD rats
SNI surgery induces neuropathic pain model in SD rats
• Efficacy testing of gabapentinoids (gabapentin, pregabalin), sodium channel blockers (lidocaine, carbamazepine, oxcarbazepine) and tricyclic antidepressants (amitriptyline, nortriptyline)
• Evaluation of novel analgesics targeting TRPV1, P2X3, and other pain pathways
• Target validation of peripheral and central sensitization mechanisms
• Biomarker discovery (pain-related neuropeptides, inflammatory mediators)
• Pharmacology and toxicology studies to support IND
scope | SNI model | saturday night live model |
Species/Strain | Sprague-Dawley Rat | |
induction method | Ligation and transection of the tibial and common peroneal branches of the sciatic nerve | Tightly ligate the L5 and/or L6 spinal nerves |
Onset and duration | Quick onset of action (within 24 hours), duration >2 months | |
sensory symptoms | Mechanical allodynia (athermal hyperalgesia) | Mechanical allodynia and thermal hyperalgesia |
critical endpoint | Mechanical Withdrawal Threshold (von Frey) | Mechanical withdrawal threshold (von Frey), thermal withdrawal latency (Hargreaves) |
| positive control | Gabapentin or pregabalin can be used as reference compounds | |
| packet | Raw data, analysis report, behavioral data (von Frey, Hargreaves), bioinformatics (optional) | |
Q: What is the difference between SNI and SNL models?
A: SNI involves ligation/transection of peripheral nerve branches, producing mechanical allodynia but no thermal hyperalgesia. SNL involves spinal nerve ligation, producing mechanical allodynia and thermal hyperalgesia. Choose SNI to conduct pure mechanical pain research; choose SNL to understand the combined mechanical/thermal pain mechanism.
Q: How is mechanical allodynia measured?
A: Using a von Frey wire, a calibrated monofilament is applied to the plantar surface of the hind paw to determine the threshold force (in grams) that elicits a withdrawal response. Lower thresholds indicate mechanical allodynia.
Q: Can these models be used for IND support studies?
Answer: Yes. Studies can be conducted according to GLP principles for regulatory submissions (FDA, EMA).
Q: Do you offer customized study protocols (e.g., prophylactic vs. therapeutic dosing, combination therapies)?
Answer: Of course. Our scientific team tailors surgical protocols, treatment plans and endpoint analyzes to your specific drug candidate.
Q: What is the typical timeline for a pilot efficacy study?
A: Behavioral assessments typically begin 7-14 days postoperatively (after recovery) and continue for 2-4 weeks for acute studies; long-term studies may extend to 8 weeks.
