Rodent Inflammatory Bowel Disease (IBD) Models

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Rodent Inflammatory Bowel Disease (IBD) Models

Rodent Inflammatory Bowel Disease (IBD) Models

Inflammatory bowel disease (IBD) encompasses chronic inflammatory conditions of the gastrointestinal tract, primarily ulcerative colitis (UC) and Crohn's disease (CD). HKeyBio offers a comprehensive panel of rodent IBD models covering multiple induction mechanisms and species: (1) DSS induced models (UC-like) in C57BL/6 mice and SD rats (acute and chronic); (2) TNBS induced models (CD-like) in C57BL/6 mice and SD rats; and (3) OXA induced models in C57BL/6, BALB/c mice, and SD rats. These models recapitulate key human IBD features including body weight loss, colon shortening, elevated DAI scores, histopathological changes, and inflammatory cytokine elevation (TNF-α, IL-6). They provide robust platforms for preclinical efficacy testing of novel anti-inflammatory and immunomodulatory therapeutics.

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Product Description

Key Features & Benefits

Broad model portfolio – DSS (UC-like), TNBS (CD-like), and OXA models covering acute, chronic, and different immune mechanisms (Th1/Th17 for TNBS, Th9 for OXA).

Multiple species/strains – C57BL/6, BALB/c mice and SD rats available.

Comprehensive endpoints – Body weight, colon length/weight, DAI score, histopathology (HE, Masson), cytokine analysis (IL-6, TNF-α), RNA-seq data (chronic DSS).

Translational value – Ideal for testing anti-inflammatory drugs, biologics (anti-TNF, anti-IL-12/23), JAK inhibitors, and gut-restricted therapies.

IND-ready data packages – Studies can be conducted in accordance with GLP principles.

Technical Data & Validation

DSS Induced Acute IBD in C57BL/6 Mice

DSS Induced Acute IBD in SD Rat

DSS Induced Chronic IBD in C57BL/6 Mice

DSS Induced Chronic IBD in SD Rat

TNBS Induced Acute IBD in C57BL/6 Mice

TNBS Induced Chronic IBD Model in C57BL/6 Mice

TNBS Induced Acute IBD in SD Rat

OXA Induced C57BL/6 IBD Model

OXA Induced BALB/c IBD Model

OXA Induced SD Rat IBD Model

Applications

• Efficacy testing of anti-inflammatory drugs (5-ASA, corticosteroids), biologics (anti-TNF, anti-IL-12/23, anti-integrin), JAK inhibitors, and S1P receptor modulators

• Evaluation of gut-restricted immunomodulators and microbiome-based therapies

• Target validation for Th1, Th17, Th9 pathways in IBD pathogenesis

• Biomarker discovery (fecal calprotectin, cytokines, inflammatory mediators)

• IND-enabling pharmacology and toxicology studies

Model Specifications

Parameter	Specification
Species/Strains	C57BL/6 mouse, BALB/c mouse, SD rat
Induction methods	DSS (oral, acute 5–7 days, chronic 2–3 cycles); TNBS (intracolonic, single or repeated); OXA (intracolonic)
Study duration	Acute: 5–10 days; Chronic: 4–8 weeks
Key endpoints	Body weight, colon length, colon weight, DAI score (0–12 scale), histopathology (HE, Masson) with scoring, cytokine levels (IL-6, TNF-α), CMDI score (TNBS), RNA-seq data (chronic DSS), colon typical pictures
Positive control	5-aminosalicylic acid (5-ASA), dexamethasone, or anti-TNF antibody available as reference compounds
Data package	Raw data, analysis reports, clinical scores, histology slides, ELISA results, RNA-seq data (optional), bioinformatics (optional)

❓ Frequently Asked Questions

Q1: What IBD models do you provide?

A1: We offer a full range of IBD models induced by DSS, TNBS and OXA, using C57BL/6, BALB/c mice and SD rats for ulcerative colitis and Crohn’s disease research.

Q2: What are the modeling mechanisms?

A2: DSS mainly induces ulcerative colitis-like lesions. TNBS mimics pathological features of Crohn’s disease. OXA triggers colitis associated with Th9 cell responses. All three agents induce intestinal inflammation and tissue damage.

Q3: What evaluation indicators are used?

A3: We detect body weight, colon length and DAI score clinically. We also test inflammatory cytokines such as IL-6 and TNF-α, and conduct HE staining for intestinal pathological assessment.

Q4: What are the experimental cycles?

A4: Acute DSS and TNBS models last around 4–9 days. The chronic DSS model runs for 29 days. The OXA-induced model is completed within 3 days after administration.

Q5: What are the advantages and applications?

A5: These models feature stable phenotypes, high reproducibility and close similarity to human IBD. They are ideal for efficacy screening, mechanism study and preclinical evaluation of anti-intestinal inflammation drugs.