| Quantity: | |
|---|---|
Clinically relevant – Recapitulates human radiation dermatitis with acute erythema, desquamation, and chronic fibrosis using standardized RTOG scoring.
Mechanism-driven – X-ray induced DNA damage, ROS production, and TGF-β mediated fibrosis mirror human radiotherapy-induced skin injury.
Comprehensive endpoints – Body weight, RTOG clinical score (0–4 scale), serum TNF-α levels, skin histopathology (HE scoring), inflammatory cell infiltration, dermal fibrosis.
Translational value – Ideal for testing radioprotectors, anti-inflammatory agents, antioxidants, and wound healing therapies.
IND-ready data packages – Studies can be conducted in accordance with GLP principles.
X-ray Induced Raddiation Dermatitis in SD Rat
• Efficacy testing of radioprotectors (amifostine, superoxide dismutase) and anti-inflammatory agents (corticosteroids, NSAIDs)
• Evaluation of wound healing agents, antioxidants, and TGF-β inhibitors for chronic radiation fibrosis
• Target validation for radiation-induced inflammatory and fibrotic pathways
• Biomarker discovery (TNF-α, TGF-β, oxidative stress markers)
• IND-enabling pharmacology and toxicology studies
Parameter | Specification |
Species/Strain | Sprague-Dawley rat |
Induction method | Local X-ray irradiation (single dose: 30–50 Gy or fractionated doses) on shaved dorsal skin, typically 2–3 cm² area |
Study duration | Acute: 1–4 weeks; Chronic: 4–12 weeks (for fibrosis studies) |
Key endpoints | Body weight, RTOG clinical score (erythema, dry/moist desquamation, ulceration, fibrosis), serum TNF-α levels (ELISA), skin histopathology (HE staining with scoring for inflammatory infiltration, epidermal thickness, dermal fibrosis), optional: immunohistochemistry (TGF-β, α-SMA, collagen I), oxidative stress markers (MDA, SOD)Body weight, RTOG clinical score (erythema, dry/moist desquamation, ulceration, fibrosis), serum TNF-α levels (ELISA), skin histopathology (HE staining with scoring for inflammatory infiltration, epidermal thickness, dermal fibrosis), optional: immunohistochemistry (TGF-β, α-SMA, collagen I), oxidative stress markers (MDA, SOD) |
| Positive control | Amifostine or corticosteroids available as reference radioprotective/anti-inflammatory compounds |
Data package | Raw data, analysis reports, clinical photographs, RTOG scoring records, ELISA results, histology slides, bioinformatics (optional) |
A1: We offer an X-ray-induced radiation dermatitis model using SD rats for preclinical research on radiation-related skin injury.
A2: X-ray radiation causes DNA damage and reactive oxygen species production in skin cells. It triggers acute inflammation and long-term TGF-β overexpression, leading to skin fibrosis, atrophy and typical radiation dermatitis lesions.
A3: We monitor body weight and assess skin lesions via RTOG scoring. We also detect TNF-α level and perform H&E staining for pathological analysis.
A4: X-ray irradiation is performed on Day 0. The whole experiment lasts 28 days until all observations and tests are completed.
