| Quantity: | |
|---|---|
Fusce pertinet - Recapitulates IIM humanam: debilitatem musculi, infiltration inflammatoriae, et cytokini pro-inflammatorii elevati (IL-6, IL-17A).
Fines quantifiabiles - pondus corporis, vires musculi (tenaci vires test), serum gradus cytokini (IL-6, IL-17A), histopathologia musculus (HE scoring), cellula infiltration inflammatoriae.
Mechanismus agitatae - Myosin immunization triggers autoimmune responsionis contra musculum sceletum, utrumque cellulosum (Th1/Th17) et immunitatem humoralem involvens myositis pathogenesis humanam.
Valor translationalis - Specimen ad probandos immunosuppressos (corticosteroides, methotrexatos, mycophenolatas), biologicos Nisl IL-6 (tocilizumab), IL-17 (secukinumab), et JAK inhibitores.
IND-parati fasciculi notati - Studia administrari possunt ad normam GLP principiorum.
Myosin inductus BALB / c DM Model
• Efficacia probatio immunosuppressantium (corticosteroides, methotrexata, mycophenolata mofetilis, cyclophosphamida)
• Aestimatio biologicorum nisl II-6 (tocilizumab), II, 17 (secukinumab, ixekizumab), et JAK inhibitoribus (tofacitinib, baricitinib)
• target sanatio pro Th17 et Th1 meatus in autoimmune myositis
• Biomarker inventio (cytokina signatura, autoantibodies, musculus damnum venalicium)
• IND - pharmacologiam et toxicologiam studiis
Parameter |
Specification |
Species/Strain |
BALB/c mus |
modus inductionis |
Immunization cum interdum myosin (eg, lepus vel myosin bubulus) in CFA emulsus, cum vel sine injectionibus cursus. |
Studium durationis |
4-8 hebdomades post-immunization (fretus morbo progressio) |
Clavis terminos |
Corpus pondus, vires musculi (tenaci vires test), serum cytokinorum gradus (II-6, IL-17A), histopathologia musculorum (HE maculat scoringum inflammationis, necrosis, regenerationis), ad libitum: cytometria fluxus musculi-influentis cellulis immunibus, anti-myosin autoantibodium. |
Data sarcina |
Rudis notitia, analysi rumores, notitia morum, histologia labitur, eventus cytokini ELISA, bioinformatici (libitum) |
A1: praebemus exemplum myosin dermatomyositis (DM) adhibitis BALB/c muribus pro inquisitione idiopathica inflammatoriae myopathiae.
A2: Myosin inductio triggers abnormes responsiones immunes, ducens ad inflammationem musculi et textus damnum. Bene recapitulat notas pathologicas myopathiae idiopathicae inflammatoriae humanae.
A3: Nos monitor corporis pondus et vires musculus amet. Etiam cytokinos inflammatorias IL-6, IL-17A deprehendimus, et IL-17A musculi pathologicam maculam geremus.
A4: Myosin administratur die 0 et die 4. Totum experimentum 28 dierum durat donec omnes probationes et observationes perficiantur.