| Rahi: | |
|---|---|
E tika ana mo te haumanu – Ko te paohu hikareti me te whakakitenga LPS he rite ki te take COPD tangata me te mumura mau tonu.
Momo maha – E waatea ana nga tauira kiore me te kiore kia rite ki nga whakaritenga whakamatautau rereke (nga taputapu ira me te rahi ake).
Nga pito mutunga - Te taumaha o te tinana, te BALF cytology (WBC, macrophages, monocytes), mahi pūkahukahu (te ātete, te tautuku), histopathology (HE scoring), cytokine profiling.
Te uara whakamaoritanga - He pai mo te whakamatautau i nga raau taero anti-inflammatory (corticosteroids, PDE4 inhibitors), bronchodilators, me te mucolytics.
Nga kohinga raraunga kua rite IND - Ka taea te whakahaere rangahau i runga i nga tikanga o te GLP.
Paowa + LPS i whakaawe Tauira COPD i C57BL/6 Kiore
Tauira COPD na te paowa i C57BL/6 Kiore
Tauira COPD i whakapourihia i roto i nga Kiore C57BL/6 whakawhiti
Paowa + LPS i whakaawe Tauira COPD i SD Rat
• Te whakamatautau kaha o nga raau taero anti-inflammatory (corticosteroids, PDE4 inhibitors, p38 MAPK inhibitors)
• Aromātai o te bronchodilators (β2-agonists, anticholinergics) me nga maimoatanga huinga
• Te whakamatautau i nga kaikawe mucoactive me nga antioxidants
• Te whakamanatanga whainga mo nga ara ahotea mumura me te waiora
• Maatauranga rongoora me te paitini whakaahei IND
Tawhā |
Tauira COPD kiore |
Tauira COPD kiore |
Momo/Riki |
C57BL/6 kiore |
Kiore Sprague-Dawley |
Tikanga whakauru |
Te rongo auahi hikareti roa (te tinana katoa, te ihu-anake ranei) 5 ra/wiki mo te 4-6 marama + intratracheal LPS (1-2 wa/wiki) |
|
Te roanga ako |
4–7 marama (whakauru + maimoatanga) |
4–7 marama (whakauru + maimoatanga) |
Nga pito mutunga matua |
Te taumaha o te tinana, te tatau pūtau BALF (tapeke me te rerekee: macrophages, monocytes, neutrophils), mahi pūkahukahu (te ātete, tautukunga, Penh), histopathology (he tohu HE mo te emphysema me te mumura), nga taumata cytokine i roto i te BALF (IL-6, TNF-α, MCP-1), he kowhiringa: tohu ahotea oxidative, me te mete-a-waea me te kopa (M. | |
Mōkihi Raraunga |
Raraunga mata, pūrongo tātaritanga, BALF cytology, raraunga mahi pūkahukahu, kiriata histology, hua ELISA, bioinformatics (kōwhiringa) | |
P: He aha te take i whakakotahi ai te paoa hikareti me te LPS ki te tauira COPD?
A: Ko te paoa hikareti ka whakapataritari i te mumura roa me te emphysema, ko te LPS (te peehi i te mate huakita) ka whakapakeke i te mumura o te hau me te hanga hūpē. Ko te whakakotahitanga ka kaha ake te whakahoki i te pathology COPD tangata, tae atu ki te whakakaha kino.
Q: He aha nga mea tino rite ki te COPD tangata?
A: Ko nga tauira e whakaatu ana i te aukati rere o te hau, te mumura o te rangi (macrophages, neutrophils), emphysema, metaplasia pūtau kaata, me nga paanga o te punaha, he rite tonu ki te COPD tangata.
P: Ka taea te whakamahi i enei tauira mo nga rangahau whakaahei IND?
A: Ae. Ka taea te whakahaere rangahau i runga i nga tikanga GLP mo nga tukunga ture (FDA, EMA).
P: Ka tukuna e koe nga tikanga ako kua whakaritea (hei tauira, he rereke te roanga o te puhipuhi auahi, nga pota LPS)?
A: Tino. Ko ta matou roopu putaiao e whakahiato i nga tikanga whakamaarama mo te auahi, te whakangao LPS, me nga tātaritanga mutunga ki to kaitono tarukino motuhake.
