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E tika ana mo te haumanu - ko te ALI i whakaawehia e te LPS he rite tonu te ahua o te ALI/ARDS i te tangata me te mumura whakapeka, te kuhu leukocyte, me te edema pulmonary.
Nga pito matawhānui - Te tatau o te pūtau BALF (lymphocytes, neutrophils, macrophages), cytokine profiling (MCP-1, IL-6, IL-10), histopathology pūkahukahu (HE, IHC), pulmonary edema assessment (makuku/maroke ōwehenga).
Ko te mahi a te miihini - Ka whakahohehia e te LPS te tohu tohu TLR4, te whakaoho i te huarahi NF-κB me te urupare inflammatory pakari, te whakaata i te whara o te pukupuku Gram-negative sepsis-induced.
Te uara o te whakamaoritanga - He pai mo te whakamatautau i nga raau taero anti-inflammatory, cytokine inhibitors, neutrophil elastase inhibitors, me nga rongoa-a-ira.
Nga kohinga raraunga kua rite IND - Ka taea te whakahaere rangahau i runga i nga tikanga o te GLP.
LPS Induced ALI Model in BALB/c Kiore
• Te whakamatautau kaha o nga raau taero anti-inflammatory (corticosteroids, NSAIDs), cytokine inhibitors (anti-IL-6, anti-TNF-α), me te neutrophil elastase inhibitors
• Aromatawai i te whakamaaramatanga o te mesenchymal stem cell (MSC) me nga maimoatanga e pa ana ki te vesicle extracellular
• Te whakamanatanga whainga mo te tohu tohu TLR4, te ara NF-κB, me nga rekereke mumura
• Te kitenga o te tohu koiora (kotaha pūtau BALF, waitohu cytokine, tohu whara pūkahukahu)
• Maatauranga rongoora me te paitini whakaahei IND
Tawhā |
Whakatakotoranga |
Momo/Riki |
BALB/c kiore |
Tikanga whakauru |
Intratracheal, intranasal, intraperitoneal whakahaere o lipopolysaccharide (LPS, 5-10 mg/kg) |
Te roanga ako |
Ake: 6–48 haora i muri i te whakahaerenga o te LPS |
Nga pito mutunga matua |
Tatau pūtau BALF (tapeke me te rerekee: neutrophils, macrophages, lymphocytes), nga taumata cytokine BALF (MCP-1, IL-6, IL-10 na ELISA), histopathology pūkahukahu (he whakapouri me te whiu whara pūkahukahu) |
Mōkihi Raraunga |
Raraunga mata, pūrongo tātari, tatau pūtau BALF, hua ELISA, kiriata hītori (HE, IHC), bioinformatics (he kōwhiri) |
A1: Ka whakatauhia e matou he tauira wharanga pukahukahu whakapeka i roto i nga kiore BALB/c na te Lipopolysaccharide (LPS).
A2: Ko te LPS te whakararu i te mumura nui o te pukahukahu, ka kuhu mai te leukocyte, te edema pulmonary me te kino ki nga arai epithelial me te endothelial pūkahukahu, e tino rite ana ki te ALI tangata.
A3: Ka kitea e matou nga lymphocytes, neutrophils me nga macrophages i roto i te BALF, ka whakamatauhia nga cytokine inflammatory tae atu ki te MCP-1, IL-6 me IL-10, ka mahi i te tae HE me te IHC mo te tātari pathological.
A4: Ka whakahaerehia te LPS hei whakararu i te whara. Ka timata te maimoatanga tarukino i te 2 haora, ka oti katoa nga tauira me nga kitenga i te 24 haora.
