| Quantity: | |
|---|---|
Clinically relevant – Mimics human postoperative pain with incisional injury, mechanical allodynia, and inflammatory response.
Quantifiable endpoints – Mechanical withdrawal threshold (von Frey filaments), spontaneous pain scoring, weight-bearing asymmetry, optional: thermal sensitivity.
Highly reproducible – Standardized surgical protocol ensures consistent pain behavior across experiments.
Translational value – Ideal for testing non-opioid analgesics, NSAIDs, local anesthetics, and novel pain management strategies.
IND-ready data packages – Studies can be conducted in accordance with GLP principles.
Surgery Incision Induced PSP Model in C57BL/6
• Efficacy testing of non-opioid analgesics (NSAIDs, COX-2 inhibitors, acetaminophen)
• Evaluation of novel analgesics including sodium channel blockers, TRPV1 antagonists, and neurokinin receptor antagonists
• Target validation for peripheral and central sensitization pathways
• Biomarker discovery (inflammatory mediators, pain-related neuropeptides)
• IND-enabling pharmacology and toxicology studies
Parameter | Specification |
Species/Strain | C57BL/6 mouse |
Induction method | Plantar incision (1 cm longitudinal incision through skin, fascia, and muscle of the hind paw) under isoflurane anesthesia |
Study duration | 1–14 days (acute pain phase) |
Key endpoints | Mechanical withdrawal threshold (von Frey filaments), spontaneous pain scoring (guarding, licking, lifting), weight-bearing asymmetry (incapacitance meter), optional: thermal hyperalgesia (Hargreaves), gait analysis, inflammatory cytokine levels (ELISA/qPCR) |
| Positive control | NSAIDs (e.g., ibuprofen, ketorolac) or gabapentin available as reference compounds |
Data package | Raw data, analysis reports, behavioral data, bioinformatics (optional) |
Q: How does surgical incision induce postoperative pain in mice?
A: The incision disrupts skin, fascia, and muscle tissues, triggering acute inflammation, peripheral sensitization, and central nervous system alterations. This results in mechanical allodynia and spontaneous pain behaviors that peak within hours and persist for days, closely mimicking human postoperative pain.
Q: What are the key similarities with human postoperative pain?
A: The model exhibits mechanical allodynia (pain from normally non-painful stimuli), spontaneous guarding behavior, and inflammatory response at the incision site, all of which are hallmarks of human postoperative pain.
Q: Can this model be used for IND-enabling studies?
A: Yes. Studies can be conducted in accordance with GLP principles for regulatory submissions (FDA, EMA).
Q: Do you offer customized study protocols (e.g., different incision sizes, pre-emptive vs. post-operative treatment)?
A: Absolutely. Our scientific team tailors incision protocols, treatment schedules (pre-emptive, post-operative), and endpoint analyses to your specific drug candidate.
Q: What is the typical timeline for a pilot efficacy study?
A: Acute pain studies are typically completed within 7 days post-incision, with behavioral testing at baseline, post-surgery, and multiple time points (e.g., 2h, 6h, 24h, 48h, 72h, 7d).
