| Quantity: | |
|---|---|
Clinically relevant – Two models capture key human EoE features: eosinophilic infiltration, Th2 inflammation, and epithelial dysfunction.
Mechanism diversity – IL-33 model reflects alarmin-driven Th2 response; OXA model represents hapten-induced allergic inflammation.
Quantifiable endpoints – Body weight, esophagus weight, serum IgE, eosinophil counts in blood and esophagus, histopathology scores (HE), granulocyte analysis.
Translational value – Ideal for testing anti-IL-5, anti-IL-13, anti-IL-33, JAK inhibitors, and steroid-sparing therapies.
IND-ready data packages – Studies can be conducted in accordance with GLP principles.
IL-33 Induced C57BL/6 EoE Model
OXA Induced C57BL/6 EoE Model
• Efficacy testing of biologics (anti-IL-5, anti-IL-13, anti-IL-33, anti-Siglec-8), JAK inhibitors, and corticosteroids
• Target validation for Th2 pathways and eosinophil recruitment
• Biomarker discovery (IgE, eosinophil-derived mediators, cytokines)
• Mechanism of action (MOA) studies for eosinophilic disorders
• IND-enabling pharmacology studies
Parameter | IL-33 Induced EoE Model | OXA Induced EoE Model |
Strain | C57BL/6 mouse | C57BL/6 mouse |
Induction method | Recombinant IL-33 (i.p. or intranasal), multiple doses over 5–14 days | Skin sensitization with OXA followed by intraluminal OXA challenge |
Study duration | 7–21 days | 10–18 days (sensitization + challenge) |
Key endpoints | Body weight, esophagus weight, serum IgE, eosinophil counts in blood and esophagus (flow cytometry), HE staining score, granulocyte analysis | Eosinophil counts in esophagus, serum IgE, HE staining score, esophageal histopathology, Th2 cytokine levels (IL-4, IL-5, IL-13) |
Data package | Raw data, analysis reports, flow cytometry files, histology slides (HE), bioinformatics (optional) | |
A1: We provide two mature C57BL/6 mouse models for Eosinophilic Esophagitis (EoE), namely the IL-33-induced model and the Oxazolone (OXA)-induced model.
A2: The IL-33-induced model triggers typical Th2 immune responses and esophageal inflammation. OXA works as a hapten to activate Th2 cells, induce cytokine secretion and massive eosinophil infiltration, both mimicking the core pathogenesis of human EoE.
A3: We test serum IgE, blood cell counts including WBC, neutrophils and eosinophils. Esophageal histopathology via HE staining is also conducted for comprehensive assessment.
A4: The IL-33-induced model takes 7 days in total. For the OXA-induced model, sensitization is performed on Day 7, followed by challenge, and the whole experiment ends on Day 28.
