| Rahi: | |
|---|---|
E whai kiko ana ki te haumanu – Ka whakahoki ano i te osteoporosis na te glucocorticoid-induced tangata me te pehi i te hanganga o nga wheua, te whakakaha ake o te resorption, me te paheketanga microarchitectural.
Momo maha – E waatea ana nga tauira kiore me te kiore kia rite ki nga hiahia whakamatautau rereke (te rahi ake me te whawhe ira).
Nga pito mutunga - Te taumaha o te tinana, te ALP serum, te atahanga X-ray, te histopathology (H&E, TRAP staining), histomorphometry wheua, micro-CT (he kōwhiringa).
Te uara o te whakamaoritanga - He pai mo te whakamatautau bisphosphonates (alendronate), SERMs (raloxifene), anabolic agents (teriparatide), me te RANKL inhibitors.
Nga kohinga raraunga kua rite IND - Ka taea te whakahaere rangahau i runga i nga tikanga o te GLP.
Dex Induced C57BL/6 Tauira Osteoporosis
Dex Induced SD Osteoporosis Tauira
• Te whakamatautau kaha o nga kaitautoko anti-osteoporotic: bisphosphonates (alendronate, zoledronic acid), SERMs (raloxifene), anabolic agents (teriparatide, romosozumab), me te RANKL inhibitors (denosumab)
• Aromatawai i nga rongoa hou e aro ana ki te hanga wheua, ki nga huarahi resorption ranei
• Te whakamanatanga o te whainga mo nga tikanga o te osteoporosis na te glucocorticoid
• Te kitenga o te tohu koiora (nga tohu hurihanga wheua, ALP, osteocalcin, CTX-I)
• Maatauranga rongoora me te paitini whakaahei IND
Tawhā |
SD Rat OP Tauira |
C57BL/6 Tauira OP Kiore |
Momo/Riki |
Kiore Sprague-Dawley |
C57BL/6 kiore |
Tikanga whakauru |
Dexamethasone (1–2.5 mg/kg) ka tukuna i roto i te uaua, i raro ranei i te kiri, 2-3 wa ia wiki mo te 4-8 wiki | |
Te roanga ako |
4–10 wiki (whakauru + wahanga maimoatanga) | |
Nga pito mutunga matua |
Te taumaha o te tinana, te ALP serum, te atahanga X-ray, te histopathology (H&E, TRAP), histomorphometry wheua, micro-CT (he kōwhiringa) |
Te taumaha o te tinana, te ALP serum, te histopathology, micro-CT (mehemea ka taea) |
| Te whakahaere pai | Ko te Alendronate me te teriparatide e waatea ana hei huinga tohutoro | |
Mōkihi Raraunga |
Raraunga mata, purongo tātaritanga, whakaahua X-ray, kiriata histology, raraunga micro-CT (kwhiria), bioinformatics (kwhiria) | |
A1: Ka whakaekea e matou nga tauira o te osteoporosis dexamethasone (Dex)-induced ma te whakamahi i nga kiore SD me nga kiore C57BL / 6 mo te rangahau preclinical mo te osteoporosis tuarua.
A2: Ko te Dexamethasone, he glucocorticoid, ka aukati i te hanganga o nga wheua me te whakararu i te paopaotanga wheua. Ka whakahekehia te papatipu wheua me te pakaru i te microarchitecture wheua, he tino whakataurite i te osteoporosis tuarua na te glucocorticoid-induced i te tangata.
A3: Ka aro turuki matou i te taumaha o te tinana, ka kitea nga taumata ALP serum, ka mahia he whakaahua X-ray me te whakakikorua H&E hei aromatawai i te hanganga wheua me nga huringa pathological.
A4: Ka whakahaerehia te Dexamethasone e rua nga wa ia wiki mai i te Ra 0. Ko nga kitenga me nga tirohanga katoa ka mahia i muri i te hanganga o te tauira.
