| Rahi: | |
|---|---|
E tika ana mo te haumanu – Ko te fibrosis i whakaawehia e CCl4 ka whakahoki ano i te fibrosis ate tangata me te hekenga o te matrix extracellular me te mate ate.
Nga pito mutunga - Te taumaha o te tinana, te serum ALT me te AST (nga tohu wharanga ate), te taatai ate (HE, Masson trichrome, Sirius Whero), te tohu fibrosis.
Ka taea te whakaputa me te tohu pai - He tauira kua whakamahia nuitia me nga kawa kua pumau, me te kaha ki te whakaputa puta noa i nga whakamatautau.
Te uara whakamaoritanga - He pai mo te whakamatautau i nga kaihoko anti-fibrotic (nintedanib, pirfenidone), antioxidants, me nga pūhui hepatoprotective.
Nga kohinga raraunga kua rite IND - Ka taea te whakahaere rangahau i runga i nga tikanga o te GLP.
CCl4 Induced C57BL/6 Tauira fibrosis ate
• Te whakamatautau whai hua o nga kaitautoko anti-fibrotic (nintedanib, pirfenidone, TGF-β inhibitors, galectin-3 inhibitors)
• Te arotake i nga raau taero hepatoprotective, antioxidants, me nga pūhui anti-inflammatory
• Te whakamanatanga whainga mo nga ara fibrosis (te whakahiato collagen, te whakahohenga pūtau stellate)
• Te kitenga o te tohu koiora (te tohu collagen, te ate enzymes, te takawaenga mumura)
• Maatauranga rongoora me te paitini whakaahei IND
Tawhā |
Whakatakotoranga |
Momo/Riki |
C57BL/6 kiore |
Tikanga whakauru |
Ko te werohanga o roto o te waro tetrachloride (CCl4, 0.5–1.0 mL/kg i roto i te hinu oriwa), 2 wa/wiki mo te 4-8 wiki |
Te roanga ako |
4–10 wiki (whakauru + wahanga maimoatanga) |
Nga pito mutunga matua |
Te taumaha o te tinana, nga taumata ALT me te AST serum (matū haumanu), histopathology ate (HE, Masson trichrome, Sirius Whero me te tohu fibrosis), he mea whiriwhiri: hydroxyproline ihirangi, serum albumin/bilirubin, immunohistochemistry (α-SMA, collagen I) |
| Te whakahaere pai | Ko te Nintedanib me te pirfenidone e waatea ana hei tohutoro pūhui anti-fibrotic |
Mōkihi Raraunga |
Raraunga mata, purongo tātaritanga, matū haumanu, kiriata histology (HE, Masson, Sirius Red), bioinformatics (he kōwhiringa) |
A1: Ka tukuna e matou te tauira ate fibrosis ate CCl₄ ma te whakamahi i nga kiore C57BL/6, he tauira preclinical matarohia mo te fibrosis ate me te rangahau cirrhosis.
A2: Ko te waro tetrachloride (CCl₄) ka mahi hei hepatotoxin. Ko ona metabolites ka pakaru i nga pūtau ate, ka whakapakeke i te mumura me te hunanga o nga cytokines profibrotic, ka mutu ka whakapakeke i te fibrosis ate.
A3: Ka aro turukihia te taumaha o te tinana, ka kitea nga taumata ALT me te AST serum mo te aromatawai mahi ate, me te tātari i te fibrosis ate me te kuhu mate mate ma te tirotiro pathological.
A4: Ka tukuna te CCl₄ kia rua nga wa ia wiki timata mai i te Ra 0. Ka patua nga kararehe ki te waahi mutunga hei whakaoti i nga kitenga katoa.