| Rahi: | |
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Ko te kōpaki tauira whanui - Mahorahora (MRL/lpr), matū (pristane), TLR-driven (imiquimod), antigen-driven (ALD-DNA, apoptotic cell), me nga tauira tangata.
Nga taumahatanga maha - MRL/lpr, C57BL/6, BALB/c, me nga kiore tangata e waatea ana.
Nga pito mutunga - Te taumaha o te tinana, nga tohu lymphadenopathy/spleen/whatuwhatu, anti-dsDNA, proteinuria, serum CREA/LDH/AST, renal histopathology (HE, IgG deposition), flow cytometry (B cells, plasma cells, T cells).
Te uara whakamaoritanga - He pai mo te whakamatautau i nga immunosuppressants, koiora (anti-CD20, anti-IFNAR), aukati TLR, me nga rongoa-a-waa-B.
Nga kohinga raraunga kua rite IND - Ka taea te whakahaere rangahau i runga i nga tikanga o te GLP.
Tauira SLE mahorahora ki MRL/lpr Kiore
Tauira SLE mahorahora i TREX1 -/- Kiore
Pristane Induced C57BL/6 SLE Model
TLR-7 Agonist Induced SLE tauira i C57BL/6 Kiore
TLR-7 Agonist Induced SLE tauira i C57BL/6 Kiore
TLR agonist i whakaawe tauira SLE tangata
ALD-DNA Tauira BALB/c SLE
Apoptosis pūtau Whakaawea te tauira BALB/c SLE
• Te whakamatautau whai hua o nga immunosuppressants (cyclophosphamide, mycophenolate, corticosteroids) me te koiora (anti-CD20, anti-BAFF, anti-IFNAR)
• Te Aromatawai o nga aukati TLR7/9, nga aukati JAK, me nga rongoa-a-waa B
• Te whakamanatanga whainga mo te hanga autoantibody, te waitohu interferon momo I, me nga huarahi nephritis
• Te kitenga tohu koiora (anti-dsDNA, proteinuria, hainatanga cytokine)
• Maatauranga rongoora me te paitini whakaahei IND
Tawhā |
Whakatakotoranga |
Nga momo/Rangi |
MRL/lpr, C57BL/6, BALB/c, kiore tangata |
Nga tikanga whakauru |
Mahorahora (Fas mutation); ip pai; te imiquimod o runga (TLR-7 agonist); kano kano kano ki te ALD-DNA, nga pūtau apoptotic ranei |
Te roanga ako |
Mahorahora: 12–20 wiki; whakaawe: 4–16 wiki i runga i te tauira |
Nga pito mutunga matua |
Te taumaha o te tinana, nga tohu lymphadenopathy/spleen/whatuwhatu, serum anti-dsDNA antibodies, proteinuria, serum CREA/LDH/AST, renal histopathology (HE, IgG/IgM deposition), flow cytometry (B, plasma cells, T cells), momo I te waitohu interferon (whakapuaki ISG |
| Te whakahaere pai | Ko te Cyclophosphamide me te mycophenolate mofetil e waatea ana hei puhui tohutoro |
| Mōkihi Raraunga | Raraunga mata, purongo tātaritanga, matū haumanu, kiriata histology, rere cytometry files, bioinformatics (kōwhiringa) |
A1: Ka whakaratohia e matou he momo tauira SLE katoa, tae atu ki te MRL/lpr tauira mahorahora, Pristane, TLR-7 agonist, ALD-DNA me nga tauira apopototic cell indud model with C57BL/6 me BALB/c kiore. Ka tukuna ano e matou he tauira kiore a TLR-7 agonist i aratakina.
A2: Ko nga kiore MRL/lpr mahorahora te whakawhanake i te mate autoimmunity. Ko nga tauira kua whakaawehia ka whakaohooho i te aukati mate kino, te nui o te hanga autoantibody me te tuunga uaua aukati, e tino whakahoki ana i nga ahuatanga pathological o te SLE tangata.
A3: Ka aroturuki matou i te taumaha o te tinana, nga tohu okana me te lymphadenopathy. Ka whakamatauhia e matou nga autoantibodies anti-dsDNA me te proteinuria, ka tātari i nga waahanga iti o te pūtau aukati, ka mahia he whakakanohi HE mo te arowhai mate whatukuhu me te ngakau.
A4: Ka kitea te tauira MRL/lpr mai i te Wiki 10 ki te Wiki 22. Ko te tauira Pristane e 26 wiki, 12 wiki te roa o te tauira agonist TLR-7, ka 12 wiki te roa o te tauira whakauru ALD-DNA.
